Direct microbial conversion
AbstractProcess development is reviewed for ethanol production from cellulosic biomass via direct microbial conversion (DMC). Experimental data addressing cellulase production and ethanol tolerance are...
View ArticleContinuous fermentation of cellulosic biomass to ethanol
AbstractExperimental results are presented for continuous conversion of pretreated hardwood flour to ethanol. A simultaneous saccharification and fermentation (SSF) system comprised ofTrichoderma...
View ArticleKinetics of the extracellular cellulases of Clostridium thermocellum acting...
AbstractInitial hydrolysis rates were examined for mixed hardwood flour pretreated with 1% sulfuric acid for 9 s at 220 °C (PTW220) and Avicel. Linear rates were observed for fractional conversion...
View ArticleRestriction endonuclease activity in Clostridium thermocellum and Clostridium...
AbstractClostridium thermocellum cell extracts exhibit specific endonuclease activity with very little non-specific exonuclease activity at 55°C. The Dam methylation system of Escherichia coli offers...
View ArticleElectrotransformation ofClostridium thermosaccharolyticum
AbstractTransformation of the thermophileClostridium thermosaccharolyticum ATCC 31960 was achieved using plasmid pCTC1 and electroporation. Evidence supporting transformation was provided by Southern...
View ArticleHot Liquid Water Pretreatment of Lignocellulosics at High Solids Concentrations
AbstractRapid (120 s), batch, hot-liquid-water pretreatment of biomass (up to 30 g) at high solids concentrations has been studied using feed materials representative of woody and herbaceous species,...
View ArticleSuperiority of the PCR-based approach for cloning the acetate kinase gene of...
Cloning of Clostridium thermocellum acetate kinase (ack) and/or phosphotransacetylase (pta) genes in Escherichia coli by functional complementation of ack and/or pta mutants was complicated by an...
View ArticleCloning of l-lactate dehydrogenase and elimination of lactic acid production...
AbstractThe gene encoding l-lactate dehydrogenase from Thermoanaerobacterium saccharolyticum JW/SL-YS485 was cloned, sequenced, and used to obtain an l-ldh deletion mutant strain (TD1) following a...
View ArticleBiosynthesis of radiolabeled cellodextrins by the Clostridium thermocellum...
AbstractThe Clostridium thermocellum cellobiose and cellodextrin phosphorylases (glucosyl transferases) in the cell extract were used to synthesize radiolabeled cellodextrins with a degree of...
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